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Image Search Results
Journal: eLife
Article Title: Serotonin modulates infraslow oscillation in the dentate gyrus during Non-REM sleep
doi: 10.7554/elife.100196
Figure Lengend Snippet: Figure 6. Genetic knockdown of Htr1a receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing
Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain (C57BL/6 J) C57BL/6 J mouse JAX: #000664 RRID:IMSR_JAX:000664 Strain (Dock10Cre) Dock10Cre mouse MGI:6117432 N/A transgene insertion, by Susumu Tonegawa Strain (Drd2Cre) Drd2Cre mouse GENSAT MMRRC:032108- UCD Strain (Slc6a4Cre) Slc6a4Cre mouse JAX: #014554 RRID:IMSR_JAX:014554 Common name: SERT- Cre Strain (Htr1aflox/flox) Htr1aflox/flox mouse Rene Hen at Columbia University N/A Generated by Rene Hen Strain (pAAV.Syn.Flex.GCaMP6s.WPRE.SV40) AAV1- SYN- FLEX- GCaMP6s Addgene RRID:Addgene_100845 Strain (AAV.CamKII.GCaMP6s.WPRE.SV40) AAV9- CamKIIa- GCaMP6s Addgene RRID:Addgene_107790 Strain (AAVdj- syn- jGCaMP7b) AAVdj- syn- jGCaMP7b Gene Vector and Virus Core at Stanford University RRID:Addgene_104489 Custom- made at Stanford virus core Strain (AAV9- hSyn- GRAB5- HT2h) AAV9- hSyn- GRAB5- HT2h Vigene Biosciences Cat#YL10097- AV9 Strain (pENN.AAV.hSyn.Cre.WPRE.hGH) AAV1- hSyn- Cre Addgene RRID:Addgene_105553 Strain (pENN.AAV.CamKII.HI.GFP- Cre.WPRE.SV40) AAV9- CaMKII- Cre- GFP Addgene RRID:Addgene_105551 Strain (pENN.AAV.CamKII0.4.eGFP.WPRE.rBG) AAV9- CaMKII- GFP Addgene
Techniques: Knockdown, Injection, Control, Transformation Assay, Activity Assay